From Causes to Consequences: Decoding Off-Target Events in CRISPR Gene Editing

• Why direct break detection matters: editing-induced DNA breaks link to downstream mutational outcomes, making direct DSB detection essential for safety assessment
• Why current methods fall short: indirect readouts, no therapeutic cell context, PCR bias, and an inability to separate editing-induced breaks from background damage
• How INDUCE-seq® solves this: PCR-free, in situ break-labelling with NGS for a sensitive, unbiased, single-nucleotide-resolution readout of on- and off-target sites directly in any therapeutically relevant cell 
• One workflow, full landscape: works across cell types, nuclease systems, and base editing modalities
• Regulatory alignment: INDUCE-seq supports the FDA's April 2026 draft guidance on NGS-based off-target assessment for IND applications

Speakers:

Beena Murthy

Senior Field Application Scientist

Broken String Biosciences