Beena Murthy
Senior Field Application Scientist Broken String Biosciences
Seminars
Tuesday 29th September 2026
From Causes to Consequences: Decoding Off-Target Events in CRISPR Gene Editing
10:00 am
- Why direct break detection matters: editing-induced DNA breaks link to downstream mutational outcomes, making direct DSB detection essential for safety assessment
- Why current methods fall short: indirect readouts, no therapeutic cell context, PCR bias, and an inability to separate editing-induced breaks from background damage
- How INDUCE-seq® solves this: PCR-free, in situ break-labelling with NGS for a sensitive, unbiased, single-nucleotide-resolution readout of on- and off-target sites directly in any therapeutically relevant cell
- One workflow, full landscape: works across cell types, nuclease systems, and base editing modalities
- Regulatory alignment: INDUCE-seq supports the FDA's April 2026 draft guidance on NGS-based off-target assessment for IND applications
